This series of pulsation vacuum sterilizer uses saturated steam as its sterilization medium. The saturated steam releases a large amount of latent heated physical properties in the stage of condensation, which presents items to be sterilized with a state of high temperature and humidity .After a period of insulation in such status,sterilization is achieved.The application of pulsating vacuum exhausting has successfully eliminatede temperature influence from cold air,and helping the sterilization items to be dried by means of vacuum and dehumidification between the jacketed layers.